4ԼɫӦ
չ⼰£ֽͨ߷Ӧ۲üԼɫ仯ڼͪ໯һ塣Уðֵɫ仯ÿ漴ȥ̼ˮҺֵɫÿȴɫ
⣬üԼķӦɰijЩṹ磺
ٶͪڼҺпתӦ칹塪ͪ໯,Գȡɫ
ͪͲͪᡢµĽṹ
ڻͪڼҺȳʻɫͨΪɫݴ˿ͪ
ۻͪ໯ﵱڶǻȡ3, 4-ǻȡʱ,ڼҺвȶױɻɫɫɫ
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1. ͪ໯һȡȡÔṹ֮Ĺϵ
1ܼȡ ûͪ໯ʼԲͬѡòܼͬȡɴﵽƴĿġ
2ȡ ͪһԣˮȴˮڼˮʿüˮȡٽˮȡҺԣͪ༴ɳ˷У«ƤաȡӦ
üᷨȡʱӦעüҺŨȲ˹ߣǿ£ʱƻͪĸˡڼữʱҲ˹ǿɪΣʹĻͪ໯ܽ⣬ͲƷʡ
3Ì¿
Ҫľƹֲͨļ״ȡУִμ̿裬ãֱԼҺ޻ͪӦʱΪֹ˹ռ̿ĩ÷ˮм״7%/ˮ15%/Һϴѡ
2. ͪ໯Ҫ뷽
1ɫ׷ ͪ໯ﳣõй轺άط۵ȡ⣬Ҳþȡ
轺ɫ ˷Ӧ÷Χ㣬Ҫڷͪͪͪ߶ȼ׻ѻĻͪͪࡣ
ɫ Էͪ໯˵ǽΪǿҪȡڻͪ໯ǻĿλüܼͪ໯֮γ޺ĴСͪ໯Ӿϴʱɣ
ԪͬϴȺ˳һա˫աաԪ
ĸǻϴٶȼӦǻĿͬʱǻλöҲӰ죬Դʻλλǻλǻϴ˳Ϊλǻͪжλλǻͪ
ͬͻͪȺ˳һͪͪͪͪ
зˡ˫ױʲͪӦĶͪϴѡ
ϾSephadex gelɫ ڻͪ໯ķ룬ҪͺŵSephadex-GͼSephadex-LH20͡
Ͼͪ໯Ļƣ
ͪʱҪáԻͪ໯̶ȡǻĿͪʱɸáϴʱͪǰɴС˳壬
2ݶpHȡ
ݶpHȡʺǿͬĻͪԪķ롣ݻͪԪǻĿλòͬǿҲͬʣԽлܼѣ5%NaHCO35%Na2CO30.2%NaOH4%NaOHҺȡﵽĿġһɴ£
Ô£ 7, 4- OH 7-4-OH Ò»OH 5-OH
NaHCO3 Na2CO3 ڲͬŨȵNaOH
3ݷijЩضŽз
ڻͪɷֵĻУڶǻɷ޴˽ṹijɷ֣Ǧη롣ڶǻijɷֿɱǦڶǻijɷֿɱʽǦݴ˿ɽɷַ롣ͪɷֻʵķȻճҺлᡢʡȣڶǻʵȣʱҲɱǦﵽȥʵĿġͪ໯Ǧɵij˼󰴳ҴУͨH2Sиֽ⣬˳ǦҺпɵõͪ໯
ġͪ໯ҩʵ
1. ΪȽⶾҩҪЧɷֻվпãΪɫ뾧ˮڼ״Ҵʱױˮʡ
2. Ҫͪ໯ܻͪӹ״Ѫļá
3. ҶĻͪ໯лͪͪա˫ͪͶصȡŹ״ѪܺѪá
塢ṹⶨ
1. ͪ໯ɫ׼
1ֽɫףPCڷȻͪ໯PĻԻͪ˵һһչijִܼҪǸݷÔз롣ڶչܼúˮҺҪǸÔз롣
2轺ɫ ڷԻͪ໯Ϻá
3ɫ ÷ΧϹ㣬رʺڷ뺬ǻĻͪࡣھԻͪ໯ǿչҪǿļԡڴչкдˮ